MGMT, GATA6, CD81, DR4, and CASP8 gene promoter methylation in glioblastoma

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MGMT promoter methylation in Peruvian patients with glioblastoma

Purpose O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation predicts the outcome and response to alkylating chemotherapy in glioblastoma. The aim of this study is to evaluate the prevalence of MGMT methylation in Peruvian glioblastoma cases. Patients and methods We evaluated retrospectively 50 cases of resected glioblastoma during the period 2008-2013 at Instituto Nacional de E...

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Detection of MGMT promoter methylation in glioblastoma using pyrosequencing.

Recent clinical trials on patients with glioblastoma revealed that O(6)-Methylguanine-DNA methyltransferase (MGMT) methylation status significantly predicts patient's response to alkylating agents. In this study, we sought to develop and validate a quantitative MGMT methylation assay using pyrosequencing on glioblastoma. We quantified promoter methylation of MGMT using pyrosequencing on paraffi...

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Personalized treatment strategies in glioblastoma: MGMT promoter methylation status

The identification of molecular genetic biomarkers considerably increased our current understanding of glioma genesis, prognostic evaluation, and treatment planning. In glioblastoma, the most malignant intrinsic brain tumor entity in adults, the promoter methylation status of the gene encoding for the repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) indicates increased efficacy of cu...

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Correlation of MGMT promoter methylation status with gene and protein expression levels in glioblastoma

OBJECTIVES 1) To correlate the methylation status of the O6-methylguanine-DNA-methyltransferase (MGMT) promoter to its gene and protein expression levels in glioblastoma and 2) to determine the most reliable method for using MGMT to predict the response to adjuvant therapy in patients with glioblastoma. BACKGROUND The MGMT gene is epigenetically silenced by promoter hypermethylation in glioma...

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Extent and patterns of MGMT promoter methylation in glioblastoma- and respective glioblastoma-derived spheres.

PURPOSE Quantitative methylation-specific tests suggest that not all cells in a glioblastoma with detectable promoter methylation of the O6-methylguanine DNA methyltransferase (MGMT) gene carry a methylated MGMT allele. This observation may indicate cell subpopulations with distinct MGMT status, raising the question of the clinically relevant cutoff of MGMT methylation therapy. Epigenetic silen...

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ژورنال

عنوان ژورنال: BMC Cancer

سال: 2012

ISSN: 1471-2407

DOI: 10.1186/1471-2407-12-218